Coding
Part:BBa_K4388003:Design
Designed by: Tomas Lopes Group: iGEM22_KCL_UK (2022-10-05)
Vitis vinifera Stilbene Synthase Mutant (VvSTS T50I/V170A)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence was codon-optimized for E.coli K12 strain.
Source
Yan, Z.-B., Liang, J.-L., Niu, F.-X., Shen, Y.-P., & Liu, J.-Z. (2021). Enhanced Production of Pterostilbene in Escherichia coli Through Directed Evolution and Host Strain Engineering. Frontiers in Microbiology, 12. doi:10.1186/s12934-017-0644-6
Mutations added to wild-type sequence from GenBank code: DQ459351.